Genome-wide fitness and expression profiling implicate Mga2 in adaptation to hydrogen peroxide.

ABSTRACT: Caloric restriction extends lifespan, an effect once thought to involve attenuation of reactive oxygen species (ROS) generated by aerobic metabolism. However, recent evidence suggests that caloric restriction may in fact raise ROS levels, which in turn provides protection from acute doses of oxidant through a process called adaptation. To shed light on the molecular mechanisms of adaptation, we designed a series of genome-wide deletion fitness and mRNA expression screens to identify genes involved in adaptation to hydrogen peroxide. Combined with known transcriptional interactions, the integrated data implicate Yap1 and Skn7 as central transcription factors of both the adaptive and acute oxidative responses. They also identify the transcription factors Mga2 and Rox1 as active exclusively i

SUBMITTER: Kelley R 

PROVIDER: S-EPMC2676504 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature