Investigation of the biophysical and cell biological properties of ferroportin, a multipass integral membrane protein iron exporter.

ABSTRACT: Ferroportin is a multipass membrane protein that serves as an iron exporter in many vertebrate cell types. Ferroportin-mediated iron export is controlled by the hormone hepcidin, which binds ferroportin, causing its internalization and degradation. Mutations in ferroportin cause a form of the iron overload hereditary disease hemochromatosis. Relatively little is known about ferroportin's properties or the mechanism by which mutations cause disease. In this study, we expressed and purified human ferroportin to characterize its biochemical/biophysical properties in solution and conducted cell biological studies in mammalian cells. We found that purified detergent-solubilized ferroportin is a well-folded monomer that binds hepcidin. In cell membranes, the N- and C-termini were both cytosolic,

SUBMITTER: Rice AE 

PROVIDER: S-EPMC2677177 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature