Meta-analysis of small RNA-sequencing errors reveals ubiquitous post-transcriptional RNA modifications.

ABSTRACT: Recent advances in DNA-sequencing technology have made it possible to obtain large datasets of small RNA sequences. Here we demonstrate that not all non-perfectly matched small RNA sequences are simple technological sequencing errors, but many hold valuable biological information. Analysis of three small RNA datasets originating from Oryza sativa and Arabidopsis thaliana small RNA-sequencing projects demonstrates that many single nucleotide substitution errors overlap when aligning homologous non-identical small RNA sequences. Investigating the sites and identities of substitution errors reveal that many potentially originate as a result of post-transcriptional modifications or RNA editing. Modifications include N1-methyl modified purine nucleotides in tRNA, potential deamination or base s

SUBMITTER: Ebhardt HA 

PROVIDER: S-EPMC2677864 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature