Regulatory targets of quorum sensing in Vibrio cholerae: evidence for two distinct HapR-binding motifs.

ABSTRACT: The quorum-sensing pathway in Vibrio cholerae controls the expression of the master regulator HapR, which in turn regulates several important processes such as virulence factor production and biofilm formation. While HapR is known to control several important phenotypes, there are only a few target genes known to be transcriptionally regulated by HapR. In this work, we combine bioinformatic analysis with experimental validation to discover a set of novel direct targets of HapR. Our results provide evidence for two distinct binding motifs for HapR-regulated genes in V. cholerae. The first binding motif is similar to the motifs recently discovered for orthologs of HapR in V. harveyi and V. vulnificus. However, our results demonstrate that this binding motif can be of variable length in V. ch

SUBMITTER: Tsou AM 

PROVIDER: S-EPMC2677876 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature