Practical and reliable FRET/FLIM pair of fluorescent proteins.

ABSTRACT:

Background

In spite of a great number of monomeric fluorescent proteins developed in the recent years, the reported fluorescent protein-based FRET pairs are still characterized by a number of disadvantageous features, complicating their use as reporters in cell biology and for high-throughput cell-based screenings.

Results

Here we screened some of the recently developed monomeric protein pairs to find the optimal combination, which would provide high dynamic range FRET changes, along with high pH- and photo-stability, fast maturation and bright fluorescence, and reliable detection in any fluorescent imaging system. Among generated FRET pairs, we have selected TagGFP-TagRFP, combining all the mentioned desirable characteristics. On the basis of this highly efficient FRET pair

SUBMITTER: Shcherbo D 

PROVIDER: S-EPMC2678114 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature