Comparative metagenomics of Daphnia symbionts.

ABSTRACT:

Background

Shotgun sequences of DNA extracts from whole organisms allow a comprehensive assessment of possible symbionts. The current project makes use of four shotgun datasets from three species of the planktonic freshwater crustaceans Daphnia: one dataset from clones of D. pulex and D. pulicaria and two datasets from one clone of D. magna. We analyzed these datasets with three aims: First, we search for bacterial symbionts, which are present in all three species. Second, we search for evidence for Cyanobacteria and plastids, which had been suggested to occur as symbionts in a related Daphnia species. Third, we compare the metacommunities revealed by two different 454 pyrosequencing methods (GS 20 and GS FLX).

Results

In all datasets we found evidence for a large number of

SUBMITTER: Qi W 

PROVIDER: S-EPMC2678164 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature