Exploring functional beta-cell heterogeneity in vivo using PSA-NCAM as a specific marker.

ABSTRACT:

Background

The mass of pancreatic beta-cells varies according to increases in insulin demand. It is hypothesized that functionally heterogeneous beta-cell subpopulations take part in this process. Here we characterized two functionally distinct groups of beta-cells and investigated their physiological relevance in increased insulin demand conditions in rats.

Methods

Two rat beta-cell populations were sorted by FACS according to their PSA-NCAM surface expression, i.e. beta(high) and beta(low)-cells. Insulin release, Ca(2+) movements, ATP and cAMP contents in response to various secretagogues were analyzed. Gene expression profiles and exocytosis machinery were also investigated. In a second part, beta(high) and beta(low)-cell distribution and functionality were investigated i

SUBMITTER: Karaca M 

PROVIDER: S-EPMC2679208 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature