Extracellular matrix modifications at fertilization: regulation of dityrosine crosslinking by transamidation.

ABSTRACT: Fertilization is accompanied by the construction of an extracellular matrix that protects the new zygote. In sea urchins, this structure is built from glycoproteins residing at the egg surface and in secretory vesicles at the egg cortex. Four enzymatic activities are required for the transformation of these proteins into the mechanically and chemically resilient fertilization envelope: proteolysis, transamidation, NADPH-dependent oxidation and peroxidation. Here, we identify the Strongylocentrotus purpuratus enzymes responsible for the formation of epsilon(gamma-glutamyl)lysine crosslinks (transamidation). We find that these two transglutaminases are activated by local acidification and act on specific substrates within the fertilization envelope (including ovoperoxidase, rendezvin and SFE

SUBMITTER: Wong JL 

PROVIDER: S-EPMC2680108 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature