Cochlin expression in anterior segment organ culture models after TGFbeta2 treatment.

ABSTRACT:

Purpose

To determine the effect of transforming growth factor (TGF)-beta2 treatment on intraocular pressure (IOP), outflow facility, and cochlin expression in vitro in monkey and pig organ-cultured anterior segments (MOCAS and POCAS).

Methods

MOCAS (rhesus and cynomolgus) or POCAS were infused with media containing 10 ng/mL TGFbeta2 to one segment of each pair and 0.1% BSA (vehicle) to the contralateral segment for up to 14 days at a constant rate. Cochlin expression was determined by immunohistochemical study, ELISA, and Western blot analysis using chicken polyclonal antibodies against different regions of cochlin.

Results

TGFbeta2 infusion produced elevated IOP in MOCAS (usually after 5 days), that was approximately 45% greater than baseline and compared to control

SUBMITTER: Bhattacharya SK 

PROVIDER: S-EPMC2680191 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature