Sizing large proteins and protein complexes by electrospray ionization mass spectrometry and ion mobility.

ABSTRACT: Mass spectrometry (MS) and ion mobility with electrospray ionization (ESI) have the capability to measure and detect large noncovalent protein-ligand and protein-protein complexes. Using an ion mobility method of gas-phase electrophoretic mobility molecular analysis (GEMMA), protein particles representing a range of sizes can be separated by their electrophoretic mobility in air. Highly charged particles produced from a protein complex solution using electrospray can be manipulated to produce singly charged ions, which can be separated and quantified by their electrophoretic mobility. Results from ESI-GEMMA analysis from our laboratory and others were compared with other experimental and theoretically determined parameters, such as molecular mass and cryoelectron microscopy and X-ray cryst

SUBMITTER: Kaddis CS 

PROVIDER: S-EPMC2680693 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature