Inferential optimization for simultaneous fitting of multiple components into a CryoEM map of their assembly.

ABSTRACT: Models of macromolecular assemblies are essential for a mechanistic description of cellular processes. Such models are increasingly obtained by fitting atomic-resolution structures of components into a density map of the whole assembly. Yet, current density-fitting techniques are frequently insufficient for an unambiguous determination of the positions and orientations of all components. Here, we describe MultiFit, a method used for simultaneously fitting atomic structures of components into their assembly density map at resolutions as low as 25 A. The component positions and orientations are optimized with respect to a scoring function that includes the quality-of-fit of components in the map, the protrusion of components from the map envelope, and the shape complementarity between pairs

SUBMITTER: Lasker K 

PROVIDER: S-EPMC2680734 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature