Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer.

ABSTRACT:

Background

With the goal of improving yield and success rates of heterologous protein production for structural studies we have developed the database and algorithm software package Gene Composer. This freely available electronic tool facilitates the information-rich design of protein constructs and their engineered synthetic gene sequences, as detailed in the accompanying manuscript.

Results

In this report, we compare heterologous protein expression levels from native sequences to that of codon engineered synthetic gene constructs designed by Gene Composer. A test set of proteins including a human kinase (P38alpha), viral polymerase (HCV NS5B), and bacterial structural protein (FtsZ) were expressed in both E. coli and a cell-free wheat germ translation system. We also compa

SUBMITTER: Raymond A 

PROVIDER: S-EPMC2680836 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature