Efficient CPP-mediated Cre protein delivery to developing and adult CNS tissues.

ABSTRACT:

Background

Understanding and manipulating gene function in physiological conditions is a major objective for both fundamental and applied research. In contrast to other experimental settings, which use either purely genetic or gene delivery (viral or non-viral) strategies, we report here a strategy based on direct protein delivery to central nervous system (CNS) tissues. We fused Cre recombinase with cell-penetrating peptides and analyzed the intracellular biological activity of the resulting chimerical proteins when delivered into cells endowed with Cre-mediated reporter gene expression.

Results

We show that active Cre enzymatic conjugates are readily internalized and exert their enzymatic activity in the nucleus of adherent cultured cells. We then evaluated this strategy i

SUBMITTER: Gitton Y 

PROVIDER: S-EPMC2680837 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature