Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues.

ABSTRACT:

Background

The concept of metabolite profiling has been around for decades and technical innovations are now enabling it to be carried out on a large scale with respect to the number of both metabolites measured and experiments carried out. However, studies are generally confined to polar compounds alone. Here we describe a simple method for lipophilic compounds analysis in various plant tissues.

Results

We choose the same preparative and instrumental platform for lipophilic profiling as that we routinely use for polar metabolites measurements. The method was validated in terms of linearity, carryover, reproducibility and recovery rates, as well as using various plant tissues.As a first case study we present metabolic profiling of Arabidopsis root and shoot tissue of wild ty

SUBMITTER: Lytovchenko A 

PROVIDER: S-EPMC2680844 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature