Novel transcripts discovered by mining genomic DNA from defined regions of bovine chromosome 6.

ABSTRACT:

Background

Linkage analyses strongly suggest a number of QTL for production, health and conformation traits in the middle part of bovine chromosome 6 (BTA6). The identification of the molecular background underlying the genetic variation at the QTL and subsequent functional studies require a well-annotated gene sequence map of the critical QTL intervals. To complete the sequence map of the defined subchromosomal regions on BTA6 poorly covered with comparative gene information, we focused on targeted isolation of transcribed sequences from bovine bacterial artificial chromosome (BAC) clones mapped to the QTL intervals.

Results

Using the method of exon trapping, 92 unique exon trapping sequences (ETS) were discovered in a chromosomal region of poor gene coverage. Sequence iden

SUBMITTER: Weikard R 

PROVIDER: S-EPMC2681481 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature