Cloning and characterization of the biosynthetic gene cluster for tomaymycin, an SJG-136 monomeric analog.

ABSTRACT: Tomaymycin produced by Streptomyces achromogenes is a naturally produced pyrrolobenzodiazepine (PBD). The biosynthetic gene cluster for tomaymycin was identified and sequenced. The gene cluster analysis reveals a novel biosynthetic pathway for the anthranilate moiety of PBDs. Gene replacement and chemical complementation studies were used to confirm the proposed biosynthetic pathway.

SUBMITTER: Li W 

PROVIDER: S-EPMC2681672 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature