Pyrosequencing of the chaperonin-60 universal target as a tool for determining microbial community composition.

ABSTRACT: We compared dideoxy sequencing of cloned chaperonin-60 universal target (cpn60 UT) amplicons to pyrosequencing of amplicons derived from vaginal microbial communities. In samples pooled from a number of individuals, the pyrosequencing method produced a data set that included virtually all of the sequences that were found within the clone library and revealed an additional level of taxonomic richness. However, the relative abundances of the sequences were different in the two datasets. These observations were expanded and confirmed by the analysis of paired clone library and pyrosequencing datasets from vaginal swabs taken from four individuals. Both for individuals with a normal vaginal microbiota and for those with bacterial vaginosis, the pyrosequencing method revealed a large number of

SUBMITTER: Schellenberg J 

PROVIDER: S-EPMC2681704 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature