Use of the lambda Red-recombineering method for genetic engineering of Pantoea ananatis.

ABSTRACT:

Background

Pantoea ananatis, a member of the Enterobacteriacea family, is a new and promising subject for biotechnological research. Over recent years, impressive progress in its application to L-glutamate production has been achieved. Nevertheless, genetic and biotechnological studies of Pantoea ananatis have been impeded because of the absence of genetic tools for rapid construction of direct mutations in this bacterium. The lambda Red-recombineering technique previously developed in E. coli and used for gene inactivation in several other bacteria is a high-performance tool for rapid construction of precise genome modifications.

Results

In this study, the expression of lambda Red genes in P. ananatis was found to be highly toxic. A screening was performed to select mutants

SUBMITTER: Katashkina JI 

PROVIDER: S-EPMC2682490 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature