A conserved salt bridge in the G loop of multiple protein kinases is important for catalysis and for in vivo Lyn function.

ABSTRACT: The glycine-rich G loop controls ATP binding and phosphate transfer in protein kinases. Here we show that the functions of Src family and Abl protein tyrosine kinases require an electrostatic interaction between oppositely charged amino acids within their G loops that is conserved in multiple other phylogenetically distinct protein kinases, from plants to humans. By limiting G loop flexibility, it controls ATP binding, catalysis, and inhibition by ATP-competitive compounds such as Imatinib. In WeeB mice, mutational disruption of the interaction results in expression of a Lyn protein with reduced catalytic activity, and in perturbed B cell receptor signaling. Like Lyn(-/-) mice, WeeB mice show profound defects in B cell development and function and succumb to autoimmune glomerulonephritis.

SUBMITTER: Barouch-Bentov R 

PROVIDER: S-EPMC2683036 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature