Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection.

ABSTRACT:

Background

Nucleofection is an emerging technology for delivery of nucleic acids into both the cytoplasm and nucleus of eukaryotic cells with high efficiency. This makes it an ideal technology for gene delivery and siRNA applications. A 96-well format has recently been made available for high-throughput nucleofection, however conditions must be optimized for delivery into each specific cell type. Screening each 96-well plate can be expensive, and descriptions of methods and outcomes to determine the best conditions are lacking in the literature. Here we employ simple methods, including cell counting, microscopy, viability and cytotoxicity assays to describe the minimal experimental methods required to optimize nucleofection conditions for a given cell line.

Methods

We compre

SUBMITTER: Bradburne C 

PROVIDER: S-EPMC2683797 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature