Highly quantitative serological detection of anti-cytomegalovirus (CMV) antibodies.

ABSTRACT:

Background

Human cytomegalovirus infection is associated with a variety of pathological conditions including retinitis, pneumonia, hepatitis and encephalitis that may be transmitted congenitally, horizontally and parenterally and occurs both as a primary infection and as reactivation in immunocompromised individuals. Currently, there is a need for improved quantitative serological tests to document seropositivity with high sensitivity and specificity.

Methods

Here we investigated whether luciferase immunoprecipitation systems (LIPS) would provide a more quantitative and sensitive method for detecting anti-CMV antibodies. Four protein fragments of immunodominant regions of CMV antigens pp150 and pp65 were generated as Renilla luciferase (Ruc) fusion proteins and used in LIPS

SUBMITTER: Burbelo PD 

PROVIDER: S-EPMC2683803 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature