Identification and characterization of new miRNAs cloned from normal mouse mammary gland.

ABSTRACT:

Background

MicroRNAs (miRNAs) are small non-coding RNAs that have been found to play important roles in silencing target genes and that are involved in the regulation of various normal cellular processes. Until now their implication in the mammary gland biology was suggested by few studies mainly focusing on pathological situations allowing the characterization of miRNAs as markers of breast cancer tumour classes. If in the normal mammary gland, the expression of known miRNAs has been studied in human and mice but the full repertoire of miRNAs expressed in this tissue is not yet available.

Results

To extend the repertoire of mouse mammary gland expressed miRNAs, we have constructed several libraries of small miRNAs allowing the cloning of 455 sequences. After bioinformatics'

SUBMITTER: Sdassi N 

PROVIDER: S-EPMC2683868 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature