Identifying genes related to choriogenesis in insect panoistic ovaries by Suppression Subtractive Hybridization.

ABSTRACT:

Background

Insect ovarioles are classified into two categories: panoistic and meroistic, the later having apparently evolved from an ancestral panoistic type. Molecular data on oogenesis is practically restricted to meroistic ovaries. If we aim at studying the evolutionary transition from panoistic to meroistic, data on panoistic ovaries should be gathered. To this end, we planned the construction of a Suppression Subtractive Hybridization (SSH) library to identify genes involved in panoistic choriogenesis, using the cockroach Blattella germanica as model.

Results

We constructed a post-vitellogenic ovary library by SSH to isolate genes involved in choriogenesis in B. germanica. The tester library was prepared with an ovary pool from 6- to 7-day-old females, whereas the drive

SUBMITTER: Irles P 

PROVIDER: S-EPMC2683872 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature