Structure and function of a Campylobacter jejuni thioesterase Cj0915, a hexameric hot dog fold enzyme.

ABSTRACT: Acyl-coenzyme A (CoA) thioesterases are a large family of enzymes that hydrolyze acyl-CoA esters to the free fatty acid and CoA and thereby regulate essential cellular functions such as lipid metabolism, membrane synthesis, signal transduction, and gene transcription. To better understand the virulence mechanisms of Campylobacter jejuni, and its possible link to membrane lipid biosynthesis, we have investigated C. jejuni thioesterases, annotated as putative proteins. While little is known about fatty acid biosynthesis and regulation in C. jejuni, remarkable differences in the genome and its organization from Escherichia coli, the paradigm system, raise questions as to the functions of these putative proteins. Here we present the crystal structure and biochemical analysis of Cj0915, definin

SUBMITTER: Yokoyama T 

PROVIDER: S-EPMC2683891 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature