Quantitative studies of the regular distribution pattern for Salmonella enteritidis in the internal organs of mice after oral challenge by a specific real-time polymerase chain reaction.

ABSTRACT:

Aim

To identify and understand the regular distribution pattern for Salmonella enteritidis (S. enteritidis) in the internal organs of mice after an oral challenge over a 3 wk period.

Methods

Assays based on the serovar-specific DNA sequence of S. enteritidis from GenBank, and a serovar-specific real-time, fluorescence-based quantitative polymerase chain reaction (FQ-PCR) were developed for the detection of S. enteritidis. We used this assay to detect genomic DNA of S. enteritidis in the blood and the internal organs, including heart, liver, spleen, kidney, pancreas, and gallbladder, from mice after oral challenge at different time points respectively.

Results

The results showed that the spleen was positive at 12 h post inoculation (PI), and the blood was at 14 h PI. T

SUBMITTER: Deng SX 

PROVIDER: S-EPMC2684009 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature