Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection.

ABSTRACT:

Background

Phaseolus vulgaris (common bean) is the second most important legume crop in the world after soybean. Consequently, yield losses due to fungal infection, like Uromyces appendiculatus (bean rust), have strong consequences. Several resistant genes were identified that confer resistance to bean rust infection. However, the downstream genes and mechanisms involved in bean resistance to infection are poorly characterized.

Results

A subtractive bean cDNA library composed of 10,581 unisequences was constructed and enriched in sequences regulated by either bean rust race 41, a virulent strain, or race 49, an avirulent strain on cultivar Early Gallatin carrying the resistance gene Ur-4. The construction of this library allowed the identification of 6,202 new bean ESTs, sig

SUBMITTER: Thibivilliers S 

PROVIDER: S-EPMC2684537 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature