Hypoxia preconditioning protection of corneal stromal cells requires HIF1alpha but not VEGF.

ABSTRACT:

Purpose

Hypoxia preconditioning protects corneal stromal cells from stress-induced death. This study determined whether the transcription factor HIF-1alpha (Hypoxia Inducible Factor) is responsible and whether this is promulgated by VEGF (Vascular Endothelial Growth Factor).

Methods

Cultured bovine stromal cells were preconditioned with hypoxia in the presence of cadmium chloride, a chemical inhibitor of HIF-1alpha, and HIF-1alpha siRNA to test if HIF-1alpha activity is needed for hypoxia preconditioning protection from UV-irradiation induced cell death. TUNEL assay was used to detect cell apoptosis after UV-irradiation. RT-PCR and western blot were used to detect the presence of HIF-1alpha and VEGF in transcriptional and translational levels.

Results

During hypoxia (

SUBMITTER: Xing D 

PROVIDER: S-EPMC2684561 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature