Prion strain discrimination based on rapid in vivo amplification and analysis by the cell panel assay.

ABSTRACT: Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay.

SUBMITTER: Karapetyan YE 

PROVIDER: S-EPMC2684634 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature