Small RNA analysis in Petunia hybrida identifies unusual tissue-specific expression patterns of conserved miRNAs and of a 24mer RNA.

ABSTRACT: Two pools of small RNAs were cloned from inflorescences of Petunia hybrida using a 5'-ligation dependent and a 5'-ligation independent approach. The two libraries were integrated into a public website that allows the screening of individual sequences against 359,769 unique clones. The library contains 15 clones with 100% identity and 53 clones with one mismatch to miRNAs described for other plant species. For two conserved miRNAs, miR159 and miR390, we find clear differences in tissue-specific distribution, compared with other species. This shows that evolutionary conservation of miRNA sequences does not necessarily include a conservation of the miRNA expression profile. Almost 60% of all clones in the database are 24-nucleotide clones. In accordance with the role of 24mers in marking repe

SUBMITTER: Tedder P 

PROVIDER: S-EPMC2685514 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature