A single C. elegans PUF protein binds RNA in multiple modes.

ABSTRACT: PUF proteins specifically bind mRNAs to regulate their stability and translation. Here we focus on the RNA-binding specificity of a C. elegans PUF protein, PUF-11. Our findings reveal that PUF-11 binds RNA in multiple modes, in which the protein can accommodate variable spacings between two distinct recognition elements. We propose a structural model in which flexibility in the central region of the protein enables the protein to adopt at least two distinct structures, one of which results in base flipping.

SUBMITTER: Koh YY 

PROVIDER: S-EPMC2685523 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature