Agrobacterium-mediated transient gene expression and silencing: a rapid tool for functional gene assay in potato.

ABSTRACT: Potato is the third most important food crop worldwide. However, genetic and genomic research of potato has lagged behind other major crops due to the autopolyploidy and highly heterozygous nature associated with the potato genome. Reliable and technically undemanding techniques are not available for functional gene assays in potato. Here we report the development of a transient gene expression and silencing system in potato. Gene expression or RNAi-based gene silencing constructs were delivered into potato leaf cells using Agrobacterium-mediated infiltration. Agroinfiltration of various gene constructs consistently resulted in potato cell transformation and spread of the transgenic cells around infiltration zones. The efficiency of agroinfiltration was affected by potato genotypes, concen

SUBMITTER: Bhaskar PB 

PROVIDER: S-EPMC2686102 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature