Characterization of an Atlantic cod (Gadus morhua) embryonic stem cell cDNA library.

ABSTRACT:

Background

The Atlantic cod is an ecologically and economically important North Atlantic fish species and also an emerging aquaculture species. To study gene expression in Atlantic cod embryonic stem (ES) cells, our goal was to generate and analyze expressed sequence tags (ESTs) from an ES cell cDNA library of mRNA consisting of approximately 3,900 ESTs.

Results

We sequenced 3,935 EST clones using a directional cDNA library made from pooled ES cells harvested at the blastula stage. Quality filtering of these ESTs allowed identification of 2,719 high-quality sequences with an average length of 442 bp containing 368 contigs and 1,276 singletons (1,644 unique sequences). BLASTX searches produced 889 significant (E-value < 10-3) hits, of which 698 (42.5%) were annotated with Gen

SUBMITTER: Olsvik PA 

PROVIDER: S-EPMC2686721 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature