Towards high-throughput molecular detection of Plasmodium: new approaches and molecular markers.

ABSTRACT:

Background

Several strategies are currently deployed in many countries in the tropics to strengthen malaria control toward malaria elimination. To measure the impact of any intervention, there is a need to detect malaria properly. Mostly, decisions still rely on microscopy diagnosis. But sensitive diagnosis tools enabling to deal with a large number of samples are needed. The molecular detection approach offers a much higher sensitivity, and the flexibility to be automated and upgraded.

Methods

Two new molecular methods were developed: dot18S, a Plasmodium-specific nested PCR based on the 18S rRNA gene followed by dot-blot detection of species by using species-specific probes and CYTB, a Plasmodium-specific nested PCR based on cytochrome b gene followed by species detection

SUBMITTER: Steenkeste N 

PROVIDER: S-EPMC2686730 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature