Measuring differential gene expression by short read sequencing: quantitative comparison to 2-channel gene expression microarrays.

ABSTRACT:

Background

High-throughput cDNA synthesis and sequencing of poly(A)-enriched RNA is rapidly emerging as a technology competing to replace microarrays as a quantitative platform for measuring gene expression.

Results

Consequently, we compared full length cDNA sequencing to 2-channel gene expression microarrays in the context of measuring differential gene expression. Because of its comparable cost to a gene expression microarray, our study focused on the data obtainable from a single lane of an Illumina 1 G sequencer. We compared sequencing data to a highly replicated microarray experiment profiling two divergent strains of S. cerevisiae.

Conclusion

Using a large number of quantitative PCR (qPCR) assays, more than previous studies, we found that neither technology is d

SUBMITTER: Bloom JS 

PROVIDER: S-EPMC2686739 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature