Global expression profiling reveals regulation of CTGF/CCN2 during lactogenic differentiation.

ABSTRACT: Mammary epithelial cells go through a series of developmental changes during pregnancy and lactation including proliferation, differentiation, secretion and apoptosis. HC11 mouse mammary epithelial cells, which undergo lactogen-induced differentiation in cell culture, were used to follow the changes in gene expression during this process. The expression profiles of over 20,000 genes were compared in HC11 cells undergoing lactogenic differentiation to non-differentiated cells using DNA microarray analysis. Greater than two fold changes were detected in 998 genes in the differentiated cells versus growth controls. Several genes including CTGF/CCN2 exhibited greater than five-fold increase. Validation of the gene expression pattern for more than twenty genes was performed. The results indicat

SUBMITTER: Wang W 

PROVIDER: S-EPMC2686753 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature