Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide.

ABSTRACT:

Aim

To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine.

Methods

The esophageal squamous carcinoma cell line (TE-13) was treated with different concentrations of nicotine (100 microg/mL and 200 microg/mL) or 200 microg/mL nicotine plus 100 micromol/L nimesulide. Cell migration and invasion were measured using migration and invasion chamber systems. COX-2 expression was determined by Western blotting. Matrix metalloproteinase-2 (MMP-2) was analyzed by zymography and ELISA.

Results

Nicotine (100 microg/mL, 200 microg/mL) enhanced TE-13 cells migration and invasion, and increased the protein expression of COX-2 and the activity of MMP-2. Nicotin

SUBMITTER: Zong Y 

PROVIDER: S-EPMC2686908 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature