Dataset Information

Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide.

ABSTRACT:

Aim

To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine.

Methods

The esophageal squamous carcinoma cell line (TE-13) was treated with different concentrations of nicotine (100 microg/mL and 200 microg/mL) or 200 microg/mL nicotine plus 100 micromol/L nimesulide. Cell migration and invasion were measured using migration and invasion chamber systems. COX-2 expression was determined by Western blotting. Matrix metalloproteinase-2 (MMP-2) was analyzed by zymography and ELISA.

Results

Nicotine (100 microg/mL, 200 microg/mL) enhanced TE-13 cells migration and invasion, and increased the protein expression of COX-2 and the activity of MMP-2. Nicotine (200 microg/mL) stimulated TE-13 cells migration and invasion which were partly blocked by nimesulide. This was associated with decreased protein expression of COX-2 and decreased activity and protein expression of MMP-2.

Conclusion

Nicotine enhances the migration and invasion of the esophageal squamous carcinoma cell line, and nimesulide partly blocks the effect of nicotine-enhanced esophageal squamous carcinoma cell migration and invasion.

SUBMITTER: Zong Y

PROVIDER: S-EPMC2686908 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature

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Publications

Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide.

Zong Ye Y Zhang Shu-Tian ST Zhu Sheng-Tao ST

World journal of gastroenterology 20090501 20

<h4>Aim</h4>To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine.<h4>Methods</h4>The esophageal squamous carcinoma cell line (TE-13) was treated with different concentrations of nicotine (100 microg/mL and 200 microg/mL) or 200 microg/mL nicotine plus 100 micromol/L nimesulide. Cell migration and invasion were measured using migration and invasion chamber systems. COX-2 ...[more]

PMID: 19469000

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Dataset Information

Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide.

Aim

Methods

Results

Conclusion

Publications

Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide.

Similar Datasets

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