Persistence of side population cells with high drug efflux capacity in pancreatic cancer.

ABSTRACT:

Aim

To investigate the persistence of side population (SP) cells in pancreatic cancer and their role and mechanism in the drug resistance.

Methods

The presentation of side population cells in pancreatic cancer cell line PANC-1 and its proportion change when cultured with Gemcitabine, was detected by Hoechst 33342 staining and FACS analysis. The expression of ABCB1 and ABCG2 was detected by real-time PCR in either SP cells or non-SP cells.

Results

SP cells do exist in PANC-1, with a median of 3.3% and a range of 2.1-8.7%. After cultured with Gemcitabine for 3 d, the proportion of SP cells increased significantly (3.8% +/- 1.9%, 10.7% +/- 3.7%, t = 4.616, P = 0.001 < 0.05). ABCB1 and ABCG2 expressed at higher concentrations in SP as compared with non-SP cells (ABCB1: 1.

SUBMITTER: Zhou J 

PROVIDER: S-EPMC2687061 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature