Gene expression induced in Escherichia coli O157:H7 upon exposure to model apple juice.

ABSTRACT: Escherichia coli O157:H7 has caused serious outbreaks of food-borne illness via transmission in a variety of food vehicles, including unpasteurized apple juice, dried salami, and spinach. To understand how this pathogen responds to the multiple stresses of the food environment, we compared global transcription patterns before and after exposure to model apple juice. Transcriptomes of mid-exponential- and stationary-phase cells were evaluated after 10 min in model apple juice (pH 3.5) using microarrays probing 4,886 open reading frames. A total of 331 genes were significantly induced upon exposure of cells to model apple juice, including genes involved in the acid, osmotic, and oxidative stress responses as well as the envelope stress response. Acid and osmotic stress response genes, includ

SUBMITTER: Bergholz TM 

PROVIDER: S-EPMC2687279 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature