Revised Mimivirus major capsid protein sequence reveals intron-containing gene structure and extra domain.

ABSTRACT:

Background

Acanthamoebae polyphaga Mimivirus (APM) is the largest known dsDNA virus. The viral particle has a nearly icosahedral structure with an internal capsid shell surrounded with a dense layer of fibrils. A Capsid protein sequence, D13L, was deduced from the APM L425 coding gene and was shown to be the most abundant protein found within the viral particle. However this protein remained poorly characterised until now. A revised protein sequence deposited in a database suggested an additional N-terminal stretch of 142 amino acids missing from the original deduced sequence. This result led us to investigate the L425 gene structure and the biochemical properties of the complete APM major Capsid protein.

Results

This study describes the full length 3430 bp Capsid coding gen

SUBMITTER: Azza S 

PROVIDER: S-EPMC2688009 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature