Analysis of lectin binding to glycolipid complexes using combinatorial glycoarrays.

ABSTRACT: Glycolipids are major components of the plasma membrane, interacting with themselves, other lipids, and proteins to form an array of heterogeneous domains with diverse biological properties. Considerable effort has been focused on identifying protein binding partners for glycolipids and the glycan specificity for these interactions, largely achieved through assessing interactions between proteins and homogenous, single species glycolipid preparations. This approach risks overlooking both the enhancing and attenuating roles of heterogeneous glycolipid complexes in modulating lectin binding. Here we report a simple method for assessing lectin-glycolipid interactions. An automatic thin-layer chromatography sampler is employed to create easily reproducible arrays of glycolipids and their heter

SUBMITTER: Rinaldi S 

PROVIDER: S-EPMC2688392 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature