Proteomic analysis of the response of the plant growth-promoting bacterium Pseudomonas putida UW4 to nickel stress.

ABSTRACT:

Background

Plant growth-promoting bacteria can alleviate the inhibitory effects of various heavy metals on plant growth, via decreasing levels of stress-induced ethylene. However, little has been done to detect any mechanisms specific for heavy metal resistance of this kind of bacteria. Here, we investigate the response of the wild-type plant growth-promoting bacterium Pseudomonas putida UW4 to nickel stress using proteomic approaches. The mutant strain P. putida UW4/AcdS-, lacking a functional 1-aminocyclopropane-1-carboxylic acid deaminase gene, was also assessed for its response to nickel stress.

Results

Two dimensional difference in-gel electrophoresis (DIGE) was used to detect significantly up- or down- regulated proteins (p < 0.05, | ratio | > 1.5) in P. putida in resp

SUBMITTER: Cheng Z 

PROVIDER: S-EPMC2689183 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature