Using in silico models to simulate dual perturbation experiments: procedure development and interpretation of outcomes.

ABSTRACT:

Background

A growing number of realistic in silico models of metabolic functions are being formulated and can serve as 'dry lab' platforms to prototype and simulate experiments before they are performed. For example, dual perturbation experiments that vary both genetic and environmental parameters can readily be simulated in silico. Genetic and environmental perturbations were applied to a cell-scale model of the human erythrocyte and subsequently investigated.

Results

The resulting steady state fluxes and concentrations, as well as dynamic responses to the perturbations were analyzed, yielding two important conclusions: 1) that transporters are informative about the internal states (fluxes and concentrations) of a cell and, 2) that genetic variations can disrupt the natural

SUBMITTER: Jamshidi N 

PROVIDER: S-EPMC2689188 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature