Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins.

ABSTRACT:

Background

Despite the powerful impact in recent years of gene expression markers like the green fluorescent protein (GFP) to link the expression of recombinant protein for selection of high producers, there is a strong incentive to develop rapid and efficient methods for isolating mammalian cell clones secreting high levels of marker-free recombinant proteins. Recently, a method combining cell colony growth in methylcellulose-based medium with detection by a fluorescently labeled secondary antibody or antigen has shown promise for the selection of Chinese Hamster Ovary (CHO) cell lines secreting recombinant antibodies. Here we report an extension of this method referred to as fluorescent labeling in semi-solid medium (FLSSM) to detect recombinant proteins significantly smaller tha

SUBMITTER: Caron AW 

PROVIDER: S-EPMC2689207 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature