Haplotype specific-sequencing reveals MBL2 association with asymptomatic Plasmodium falciparum infection.

ABSTRACT:

Background

Mannose binding lectin (MBL) has an important role in the activation of the complement system and opsonization of pathogenic microorganisms. Frequent polymorphisms found in the MBL2 gene affect the concentration and functionality of the protein and are associated with enhanced susceptibility to severe malaria in African children. Most MBL2 typing strategies were designed to the analysis of selected variants, the significance of whole haplotypes is poorly known. In this work, a new typing strategy was developed and validated in an association analysis of MBL2 with adult asymptomatic infection.

Methods

MBL2 allele-specific fragments of 144 healthy Gabonese adults were amplified by using haplotype-specific sequencing (HSS), a new strategy that combines sequence-speci

SUBMITTER: Boldt AB 

PROVIDER: S-EPMC2689254 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature