Discovering new bioactive neuropeptides in the striatum secretome using in vivo microdialysis and versatile proteomics.

ABSTRACT: The striatum, a major component of the brain basal nuclei, is central for planning and executing voluntary movements and undergoes lesions in neurodegenerative disorders such as Huntington disease. To perform highly integrated tasks, the striatum relies on a complex network of communication within and between brain regions with a key role devoted to secreted molecules. To characterize the rat striatum secretome, we combined in vivo microdialysis together with proteomics analysis of trypsin digests and peptidomics studies of native fragments. This versatile approach, carried out using different microdialysis probes and mass spectrometer devices, allowed evidencing with high confidence the expression of 88 proteins and 100 processed peptides. Their secretory pathways were predicted by in sil

SUBMITTER: Bernay B 

PROVIDER: S-EPMC2689773 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature