Identification of early intestinal neoplasia protein biomarkers using laser capture microdissection and MALDI MS.

ABSTRACT: Obtaining protein profiles from a homogeneous cell population in tissues can significantly improve our capability in protein biomarker discovery. In this study, unique protein profiles from the top and bottom sections of mouse crypts and Apc(Min+/-) adenomas were obtained using laser capture microdissection (LCM) combined with MALDI MS. Statistically significant protein peaks with differential expression were selected, and a set of novel protein biomarkers were identified. Immunohistochemistry was performed to confirm the differentially expressed protein biomarkers found by LCM combined with MALDI MS. To validate the relevance of the findings in human colorectal cancer (CRC), S100A8 was further confirmed in human CRC using immunohistochemistry. In addition, S100A8 was found to have an incr

SUBMITTER: Xu BJ 

PROVIDER: S-EPMC2689774 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature