Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium.

ABSTRACT: Proteins that exhibit different expression levels in normal and malignant lung cells are good candidate biomarkers to improve early diagnosis and intervention. We used a quantitative approach and compared the proteome of microdissected cells from normal human bronchial epithelium and squamous cell carcinoma tumors of histopathological grades G2 and G3. DIGE analysis and subsequent MS-based protein identification revealed that 32 non-redundant proteins were differentially regulated between the respective tissue types. These proteins are mainly involved in energy pathways, cell growth or maintenance mechanisms, protein metabolism, and the regulation of DNA and RNA metabolism. The expression of some of these proteins was analyzed by immunohistochemistry using tissue microarrays containing tis

SUBMITTER: Poschmann G 

PROVIDER: S-EPMC2689775 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature