Grade-dependent proteomics characterization of kidney cancer.

ABSTRACT: Kidney cancer is frequently metastatic on presentation at which point the disease is associated with a 95% mortality. Assessment of tumor grade on pathological examination is the most powerful means for prognostication as well as for stratification of patients into those who might respond to conventional or targeted therapy. Although there exist several grading systems in common use, all suffer from significant disparity among observers. In an attempt to objectify this process as well as to acquire grade-specific mechanistic information, we performed LC-MS/MS-based proteomics analysis on 50 clear cell kidney cancers equally distributed among normal tissues and Fuhrman grades 1-4. Initial experiments confirmed the utility of using archived formalin-fixed paraffin-embedded samples for LC-MS/

SUBMITTER: Perroud B 

PROVIDER: S-EPMC2689781 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature