Genome-wide identification of alternative splice forms down-regulated by nonsense-mediated mRNA decay in Drosophila.

ABSTRACT: Alternative mRNA splicing adds a layer of regulation to the expression of thousands of genes in Drosophila melanogaster. Not all alternative splicing results in functional protein; it can also yield mRNA isoforms with premature stop codons that are degraded by the nonsense-mediated mRNA decay (NMD) pathway. This coupling of alternative splicing and NMD provides a mechanism for gene regulation that is highly conserved in mammals. NMD is also active in Drosophila, but its effect on the repertoire of alternative splice forms has been unknown, as has the mechanism by which it recognizes targets. Here, we have employed a custom splicing-sensitive microarray to globally measure the effect of alternative mRNA processing and NMD on Drosophila gene expression. We have developed a new algorithm to i

SUBMITTER: Hansen KD 

PROVIDER: S-EPMC2689934 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature