Viral suppressors of RNA silencing hinder exogenous and endogenous small RNA pathways in Drosophila.

ABSTRACT:

Background

In plants and insects, RNA interference (RNAi) is the main responder against viruses and shapes the basis of antiviral immunity. Viruses counter this defense by expressing viral suppressors of RNAi (VSRs). While VSRs in Drosophila melanogaster were shown to inhibit RNAi through different modes of action, whether they act on other silencing pathways remained unexplored.

Methodology/principal findings

Here we show that expression of various plant and insect VSRs in transgenic flies does not perturb the Drosophila microRNA (miRNA) pathway; but in contrast, inhibits antiviral RNAi and the RNA silencing response triggered by inverted repeat transcripts, and injection of dsRNA or siRNA. Strikingly, these VSRs also suppressed transposon silencing by endogenous siRNAs (en

SUBMITTER: Berry B 

PROVIDER: S-EPMC2689938 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature